Journal: PLoS ONE

Article Title: Innate immune functions of avian intestinal epithelial cells: Response to bacterial stimuli and localization of responding cells in the developing avian digestive tract

doi: 10.1371/journal.pone.0200393

Figure Lengend Snippet: Micrographs A & B show a typical epithelial monolayer under phase contrast microscopy. In A, the arrow head shows the intestinal fragment from which the monolayer originated. The monolayer gradually extended and assumed a typical mosaic structure (B) typical to epithelium. These structures are further characterized by light microscopy (May-Grunwald staining) (C & D). The typical mosaic structure is seen in the central area of D. The epithelium was stained with two FITC labeled polyclonal antibodies: mouse anti-villin antibody (E) and mouse anti-E-cadherin antibody (F). Goblet cells were demonstrated by staining with either goat polyclonal antibody to Muc2 (G) followed by staining with Alexa Fluor® 488 donkey anti goat IgG (H+L) or PAS (H; left x 4, right x 40).

Article Snippet: Primary antibodies used were: mouse monoclonal anti-human secretory component (clone GA-1; Sigma-Aldrich, Inc., Israel), purified mouse or rabbit IgG anti-lysozyme prepared in our lab (Briefly, a highly purified source of hen egg white was used for immunization [L4631, Sigma-Aldrich, Israel]; the resulting antibodies did not cross react with avidin, ovalbumin or ovotransferrin), polyclonal rabbit anti-chicken avidin (Thermo-Fisher Scientific), polyclonal goat anti-Muc2 (R12) (Santa-Cruz Biotechnology, Inc., Dallas, TX, USA) Detecting (secondary) antibodies used were: HRP labeled goat anti-mouse IgG (H+L) (KPL Gaithesburg, MD, USA) or HRP labeled goat anti-rabbit IgG (H+L) (Jackson Immuno Research, Laboratories, Inc., West Grove, PA, USA) and Alexa Fluor® 488 donkey anti goat IgG (H+L) (Jackson Immuno Research, Laboratories, Inc., West Grove, PA, USA).

Techniques: Microscopy, Light Microscopy, Staining, Labeling

Journal: PLoS ONE

Article Title: Innate immune functions of avian intestinal epithelial cells: Response to bacterial stimuli and localization of responding cells in the developing avian digestive tract

doi: 10.1371/journal.pone.0200393

Figure Lengend Snippet: Two—3μm thick slides were stained by mouse anti-lysozyme and HRP-conjugated goat anti mouse IgG (H+L). A-D duodenum day 0 (x400), E-F duodenum day 0 (x1000). Arrow indications are described in Results. Negative control (secondary antibody only)—Insert in panel F.

Article Snippet: Primary antibodies used were: mouse monoclonal anti-human secretory component (clone GA-1; Sigma-Aldrich, Inc., Israel), purified mouse or rabbit IgG anti-lysozyme prepared in our lab (Briefly, a highly purified source of hen egg white was used for immunization [L4631, Sigma-Aldrich, Israel]; the resulting antibodies did not cross react with avidin, ovalbumin or ovotransferrin), polyclonal rabbit anti-chicken avidin (Thermo-Fisher Scientific), polyclonal goat anti-Muc2 (R12) (Santa-Cruz Biotechnology, Inc., Dallas, TX, USA) Detecting (secondary) antibodies used were: HRP labeled goat anti-mouse IgG (H+L) (KPL Gaithesburg, MD, USA) or HRP labeled goat anti-rabbit IgG (H+L) (Jackson Immuno Research, Laboratories, Inc., West Grove, PA, USA) and Alexa Fluor® 488 donkey anti goat IgG (H+L) (Jackson Immuno Research, Laboratories, Inc., West Grove, PA, USA).

Techniques: Staining, Negative Control